pak2 thr402 rabbit polyclonal cell signaling technology  (Cell Signaling Technology Inc)

Journal: Redox Biology

Article Title: Changes to insulin sensitivity in glucose clearance systems and redox following dietary supplementation with a novel cysteine-rich protein: A pilot randomized controlled trial in humans with type-2 diabetes

doi: 10.1016/j.redox.2023.102918

Figure Lengend Snippet: The effect of 14-weeks of CON, WHEY or KDPWHE treatment in adults with type-2 diabetes mellitus on phosphoprotein concentration within the insulin-receptor signalling pathway upstream of regulatory nodes for GLUT4 translocation within the skeletal muscle in responses to basal (INS -) and insulin stimulation (hyperinsulinemic-isoglycaemic; INS +), with data shown as relative protein abundance at week 0 and 15. (A) Insulin-receptor substrate-1 phosphorylated on Ser312 (IRS-1 Ser312 ). (B) Representative immunofluorescence images of cross-sectional skeletal muscle fibres stained for IRS-1 Ser312 . IRS-1 ser312 was measured as staining intensity in the plasma membrane region (SM ). The small grey scale lines bottom right represent 50 μm. (C) Protein kinase B phosphorylation on Ser437 (Akt Ser437 ) expressed relative to total Akt. (D) Akt substrate 160 kDa phosphorylation on Thr642 (AS160 Thr642 ), (E) p21 activated kinase (PAK) phosphorylation on isoform 1 (PAK Thr423 ) and 2 (PAK Thr402 ). Data are raw unit median, upper and lower quartiles, and range, with individual-participant data points included. Raw unit point and change score mean and SD are in SM Data 1. Outcome statistics are in . CON, non-protein isocaloric control; KDPWHE, keratin-derived protein with whey; WHEY, whey protein isolate.

Article Snippet: Blocked membranes were then incubated overnight at 4 °C with the following antibodies diluted in TBS-T/3%BSA; rabbit polyclonal anti-human PRX2 at 1:10,000 (R8656, Sigma-Aldrich, RRID: AB_1841096 ), rabbit polyclonal anti-human PRX3 at 1:10,000 (PA0030 AbFrontier, RRID: AB_1620980 ), rabbit monoclonal anti-NRF2 at 1:1, 000 (ab62352, Abcam, RRID: AB_944418 ), rabbit polyclonal anti-GPx1 at 1:1000 (11797, ThermoFisher, RRID: AB_2723218 ), mouse monoclonal anti-SOD1 at 1:1000 (MA1-105, ThermoFisher, RRID: AB_2536811 ), rabbit polyclonal anti-phospho-AS160 Thr642 at 1:1000 (4288, Cell Signalling Technology), rabbit polyclonal anti-phospho-PAK1 Thr423 /p-PAK2 Thr402 at 1:1000 (2601, Cell Signalling Technology), rabbit polyclonal anti-phospho-Akt Ser437 at 1:1000 (44-621G, ThermoFisher), rabbit monoclonal anti-Pan-Akt at 1:1000 (MA5-14916, Thermofisher), mouse monoclonal anti-α-tubulin HRP-conjugate at 1:1000 (ab40742, Abcam).

Techniques: Concentration Assay, Translocation Assay, Quantitative Proteomics, Immunofluorescence, Staining, Clinical Proteomics, Membrane, Phospho-proteomics, Control, Derivative Assay

Journal: Redox Biology

Article Title: Changes to insulin sensitivity in glucose clearance systems and redox following dietary supplementation with a novel cysteine-rich protein: A pilot randomized controlled trial in humans with type-2 diabetes

doi: 10.1016/j.redox.2023.102918

Figure Lengend Snippet: The effect of 14-weeks of KDPWHE, WHEY, or CON treatment in adults with type-2 diabetes mellitus on skeletal muscle tissue insulin signalling, capillarization, and capillary endothelial nitric oxide synthase activity.

Article Snippet: Blocked membranes were then incubated overnight at 4 °C with the following antibodies diluted in TBS-T/3%BSA; rabbit polyclonal anti-human PRX2 at 1:10,000 (R8656, Sigma-Aldrich, RRID: AB_1841096 ), rabbit polyclonal anti-human PRX3 at 1:10,000 (PA0030 AbFrontier, RRID: AB_1620980 ), rabbit monoclonal anti-NRF2 at 1:1, 000 (ab62352, Abcam, RRID: AB_944418 ), rabbit polyclonal anti-GPx1 at 1:1000 (11797, ThermoFisher, RRID: AB_2723218 ), mouse monoclonal anti-SOD1 at 1:1000 (MA1-105, ThermoFisher, RRID: AB_2536811 ), rabbit polyclonal anti-phospho-AS160 Thr642 at 1:1000 (4288, Cell Signalling Technology), rabbit polyclonal anti-phospho-PAK1 Thr423 /p-PAK2 Thr402 at 1:1000 (2601, Cell Signalling Technology), rabbit polyclonal anti-phospho-Akt Ser437 at 1:1000 (44-621G, ThermoFisher), rabbit monoclonal anti-Pan-Akt at 1:1000 (MA5-14916, Thermofisher), mouse monoclonal anti-α-tubulin HRP-conjugate at 1:1000 (ab40742, Abcam).

Techniques: Activity Assay, Phospho-proteomics

Journal: Developmental cell

Article Title: Long-range optogenetic control of axon guidance overcomes developmental boundaries and defects

doi: 10.1016/j.devcel.2020.05.009

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-Thr423 phospho-PAK1 , Cell Signaling Technology , Cat#2601T; RRID: AB_330220.

Techniques: Recombinant, TaqMan SNP Genotyping Assay, Cloning, Mutagenesis, cDNA Synthesis, Construct, Plasmid Preparation, Software, Microscopy